The study involved 1,050 college students to validate the Chinese version of the Problematic TikTok Use Scale (PTTUS), originally developed by Günlü et al. Based on the translation and back-translation of the original PTTUS, the specific items of PTTUS in Chinese were determined, and then the validity of the scale was verified again by item analysis, exploratory factor analysis, confirmatory factor analysis, criterion related validity analysis, reliability test, and cross-gender measurement invariance test. The results showed that the three-factor model of the Chinese version of the Problematic TikTok Use Scale fit well. The internal consistency reliability, test-retest reliability, construct validity, and criterion-related validity all met the standards of psychometrics. Furthermore, the scale demonstrated measurement invariance across different genders. In conclusion, the Chinese version of the Problematic TikTok Use Scale can serve as an effective tool for assessing problematic TikTok use among college students.