To investigate the influence of partner phubbing on family functioning, and the mediating role of marital relationship as well as perceived stress, a total of 959 married adults were investigated by the partner phubbing scale, Chinese perceived stress scale, marital relationship questionnaire, family adaptability and cohesion evaluation scales. The results showed that family functioning negatively correlated with partner phubbing and perceived stress, and positively correlated with marital relationship. There was a significant positive correlation between partner phubbing and perceived stress, and a significant negative correlation between partner phubbing and marital relationship. After controlling for the gender and age, the testing of serial mediation model indicated that perceived stress and marital relationship separately played a mediating role between partner phubbing and family functioning; perceived stress-marital relationship played a chain mediating role between partner phubbing and family functioning. The indirect effect size are respectively 31.58%, 19.60%, 16.55%. The results revealed the mechanism of partner phubbing on family functioning and provide guidance for the construction of family functioning.